CULTIVATION OF IN VITRO PROPAGATED KIWI (ACTINIDIA DELICIOSA) PLANTS ON DIFFERENT SUBSTRATES
Keywords:
In vitro, kiwi, explant, clonal micropropagation, apical meristem, callus tissue, cytokinin, auxin, phytohormones, acclimatization.Abstract
This article presents the results of experiments conducted on the acclimatization of kiwi (Actinidia deliciosa) plants propagated under in vitro conditions using various substrates in a laboratory setting. Among the tested variants, substrates with a high proportion of coconut peat yielded the best results. In particular, in Variant 7, which consisted of 60% coconut peat, 20% vermiculite, 10% perlite, and 10% soil, the plant survival rate reached 86%. In other variants, composed of 60% soil, 10% biohumus, 10% coconut peat, 10% vermiculite, and 10% perlite, plants grew up to an average height of 65 cm.
References
Abdullayev, A. (2021). Introduction of modern technologies and varieties in kiwi cultivation. Academy of Agriculture of Uzbekistan.
Albuquerque, P., Ribeiro, R., & Mendes, M. (2015). Kiwi plant breeding and technology improvement. Fruit Science Journal, 45(2), 120–130.
Basu, S., Ghosh, P., & Dutta, D. (2016). Effect of different concentrations of BAP and IBA on in vitro propagation of kiwifruit. International Journal of Plant Biology, 7(3), 1–6.
Camarillo, I., Valdes, J., & Garcia, F. (2016). In vitro propagation methods: Microclonal propagation in kiwi plant. Plant Cell Reports, 35(7), 1605–1612.
Chandra, R., Meena, S., & Agarwal, A. (2015). Role of plant growth regulators in in vitro propagation of horticultural crops. Science Progress, 98(2), 214–225.
Dawood, M., Al-Dosary, M., & Khalaf, N. (2017). In vitro sterilization techniques for plant tissue culture: A review. International Journal of Advanced Research, 5(3), 1737–1744.
Duan, Q., Zhang, Z., & Liu, J. (2018). Kiwi cultivation and adaptation of new varieties to climate. Agricultural Research Review, 22(1), 49–58.
Ferguson, A., Mcneil, D., & Hayward, G. (2018). Kiwi breeding: New varieties and innovative technologies. New Zealand Journal of Horticultural Science, 46(4), 350–366.
Gao, W., Zhou, X., & Wang, J. (2019). Organoleptic properties and benefits of kiwi fruits. Food Chemistry, 245, 563–572.
Hassan, M., Al-Aswad, A., & Ahmed, M. (2015). In vitro propagation and role of plant growth regulators. Biotechnology Advances, 33(6), 977–983.
Hassan, R., Iqbal, A., & Uddin, M. (2021). In vitro propagation of kiwi (Actinidia deliciosa) and its rooting response to various plant growth regulators. Plant Cell Tissue and Organ Culture, 146(1), 1–8.
Huang, Y., Zhan, D., & Chen, X. (2020). Improving disease immunity in kiwi plants. Horticultural Science, 55(3), 200–210.
Mithila, K., Shikha, K., & Rani, S. (2019). Effect of sterilization agents on in vitro cultures of medicinal plants. Plant Cell Reports, 38(4), 421–431.
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum, 15(3), 473–497.
Narayana, A., Ramesh, G., & Kiran, P. (2017). Effect of plant growth regulators on root induction and shoot multiplication of Actinidia deliciosa in vitro. Journal of Applied Horticulture, 19(2), 115–120.
Nizam, M., Arshad, M., & Saeed, T. (2014). In vitro culture of kiwifruit: Effect of media composition on regeneration and rooting. Indian Journal of Biotechnology, 13(4), 434–439.
Pshikhacheva, T. (2012). Kiwi yield and breeding processes. Journal of Fruit Production, 39(2), 121–128.
Razi, M., Shamsi, K., & Shah, R. (2020). Effect of BAP concentration on shoot proliferation and rooting of kiwi (Actinidia deliciosa) in vitro. Horticultural Science, 45(1), 22–30.
Santos, J., Kumar, R., & Patel, D. (2020). New technologies and production efficiency in kiwi cultivation. International Journal of Agricultural Research, 18(4), 235–248.
Singh, R., Sharma, M., & Patil, M. (2018). Effect of auxins and cytokinins on in vitro regeneration of Actinidia deliciosa. Acta Horticulturae, 1215, 89–95.
Yuldashev, F., Bazarov, S., & Kamolov, R. (2020). Development trends in kiwi cultivation in Uzbekistan. Journal of Horticultural Science, 45(1), 23–30.
