OPTIMIZATION OF MICROCLONAL PROPAGATION METHODS OF JUGLANS REGIA L.
Keywords:
in vitro, nutrient medium, Juglans regia L., proliferation, DKW, MS, standard error (SE).Abstract
This scientific article shows that, in the propagation of Juglans regia L., the composition of the culture medium is the main source of variability in the proliferation process. Regardless of the genotype used, the growth of microshoots was better in DKW medium than in MS medium. The results indicate that growth occurred mainly due to the elongation of internodes rather than an increase in the number of nodes. In addition, the size of calli formed on DKW medium was larger than those on MS medium. Values in each column are presented as mean ± standard error (SE). n = 20; mean values of explant shoots marked with different letters differ significantly at P < 0.05.
References
Abdalla, N., El-Ramady, H., Seliem, M. K., El-Mahrouk, M. E., Taha, N., Bayoumi, Y., Shalaby, T. A., & Dobránszki, J. (2022). An academic and technical overview on plant micropropagation challenges. Horticulturae, 8(8), 677. https://doi.org/10.3390/horticulturae8080677
Ahmadpoor, F., Zare, N., Asghari, R., & Sheikhzadeh, P. (2022). Sterilization protocols and the effect of plant growth regulators on callus induction and secondary metabolites production in in vitro cultures of Melia azedarach L. AMB Express, 12(1), 1–12.
Vahdati, K., Sadeghi-Majd, R., Sestras, A. F., Licea-Moreno, R. J., Peixe, A., & Sestras, R. E. (2022). Clonal propagation of walnuts (Juglans spp.): A review on evolution from traditional techniques to application of biotechnology. Plants, 11(22), 3040.
https://doi.org/10.3390/plants11223040
Vahdati, K., & Khalighi, A. (2001). Persian walnut stooling in Iran. Acta Horticulturae, 544, 531–535.
Driver, J. A., & Kuniyuki, A. H. (1984). In vitro propagation of Paradox walnut rootstock. HortScience, 19(4), 507–509.
Jugreet, B. S., & Mahomoodally, M. F. (2020). Essential oils from nine exotic and endemic medicinal plants from Mauritius show in vitro antibacterial and antibiotic-potentiating activities. South African Journal of Botany, 132, 355–362.
Kutas, E., & Ogorodnik, L. (2011). The influence of sterilizing compounds on the yield of viable explants of Rhododendron L. (Ericaceae). International Journal of Biodiversity and Conservation, 3(1), 24–26.
Leal, D. R., Sánchez-Olate, M., Avilés, F., Materan, M. E., Uribe, M., Hasbún, R., & Rodríguez, R. (2007). Micropropagation of Juglans regia L. In S. M. Jain & H. Häggman (Eds.), Protocols for micropropagation of woody trees and fruits (pp. 381–390). Springer.
Leslie, C., & McGranahan, G. (1992). Micropropagation of Persian walnut (Juglans regia L.). In Y. P. S. Bajaj (Ed.), High-tech and micropropagation II (pp. 136–150). Springer.
Licea-Moreno, R. J., Contreras, A., Morales, A. V., Urban, I., Daquinta, M., & Gomez, L. (2015). Improved walnut mass micropropagation through the combined use of phloroglucinol and FeEDDHA. Plant Cell, Tissue and Organ Culture, 123(1), 143–154.
Murashige, T., & Tucker, D. P. H. (1969). Growth factor requirements of citrus tissue culture. Proceedings of the First International Citrus Symposium, 1155–1161.
McGranahan, G., Leslie, C. A., & Driver, J. A. (1988). In vitro propagation of mature Persian walnut varietys. HortScience, 23(1), 220.
Singh, C. R. (2018). Review on problems and their remedies in plant tissue culture. Asian Journal of Biological Sciences, 11, 165–172.
Stevens, M. E., & Pijut, P. M. (2018). Rapid in vitro shoot multiplication of the recalcitrant species Juglans nigra L. In Vitro Cellular & Developmental Biology – Plant, 54(3), 309–317.
Sharma, S., Sharma, S., & Kumar, P. (2025). In vitro establishment of walnut (Juglans regia L.) from the embryonic axis with customized nutrient media and synergy of growth regulators. South African Journal of Botany, 177, 598–603.
Pijut, P. M. (1997). Micropropagation of Juglans cinerea L. (butternut). In Y. P. S. Bajaj (Ed.), High-tech and micropropagation V (pp. 345–357). Springer.
Yegizbayeva, T. K., García-García, S., Yausheva, T. V., Kairova, M., Apushev, A. K., Oleichenko, S. N., & Licea-Moreno, R. J. (2021). Unraveling factors affecting micropropagation of four Persian walnut varieties. Agronomy, 11(7). https://doi.org/10.3390/agronomy11071407
